4/1/2024 0 Comments Cat weight tracker templating![]() MitoTracker Red FM and MitoTracker Deep Red FM are well suited for multicolor labeling experiments because their red fluorescence is well resolved from the green fluorescence of other probes.īenefits of using MitoTracker mitochondria labeling dyes The carbocyanine-based MitoTracker Deep Red FM stains active mitochondrial in live cells and is well-retained in mitochondria after aldehyde fixation and subsequent permeabilization with detergents for applications that require subsequent processing such as immunocytochemistry or in situ hybridization. The carbocyanine-based dyes MitoTracker Green FM and MitoTracker Red FM accumulate in active mitochondria but are not well-retained in mitochondria after aldehyde fixation. MitoTrackerRed and Orange are well suited for multicolor labeling experiments because their fluorescence is well resolved from the green fluorescence of other probes.Ĭarbocyanine-based MitoTracker dyes for mitochondria labelingĬell-permeant MitoTracker dyes stain active mitochondria in live cells for mitochondrial labeling and localization in fluorescent cell imaging. ![]() The fluorescent signal from the rosamine-based MitoTracker dyes is retained in the mitochondria even after aldehyde fixation and detergent permeabilization, making these mitochondria dyes flexible for many workflows including applications that require subsequent processing such as immunocytochemistry or in situ hybridization. MitoTracker Orange CM-H2TMRos and MitoTracker Red CM-H2XRos are reduced, nonfluorescent versions of the rosamine-based MitoTracker dyes that fluoresce upon oxidation. Our rosamine-based mitochondrial staining dyes include MitoTracker Orange CMTMRos, a derivative of tetramethylrosamine, and MitoTracker Red CMXRos, a derivative of X-rosamine. ![]() Rosamine-based MitoTracker dyes for mitochondria labelingĬell-permeant MitoTracker probes stain active mitochondria in live cells for labeling and localization in fluorescent cell imaging.
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